Extensive research and development on the ADT process has already been done. Pilot plants have operated on a commercial basis over several years using a wide variety of raw material and producing a wide range of meals. Some of the products that have been trialled are conventional meat and bone meal, blood meal, poultry meal from abattoir waste and downer and dead birds, feather meal, fish meal and prawn meal. All physical engineering data are well documented and available for plant design.

Immunoblot (Western blot) experimentation has been conducted with active TSE material and in all cases no TSE could be detected in the samples processed with the ADT process. This is also supported by protein hydrolysis studies using actual material from a low temperature plant and analyzed with sodium dodecyl sulphonate polyacrylamide gel electrophoresis (SDS PAGE) and demonstrated that all protein in the samples has a residual polypeptide size of < 5 KDa. These studies have also conducted molecular sizing on experimentation with known bioassay data. These data have demonstrated the molecular sizing technique to be extremely accurate in determining TSE inactivation as in the SDS PAGE gel below:

sds page

Lane M = Markers

Lanes 31& 35 = Molecular sizing of known bioassay results with 2.5 logs reduction in TSE infectivity. Note bands of unhydrolysed protein.

Lanes 32 & 36 = Molecular sizing of known bioassay results with 3.6 logs reduction in TSE infectivity. Note bands of unhydrolysed protein.

Lanes 33, 37, 34 & 38 = Molecular sizing of known bioassay results with > 6 logs reduction in TSE infectivity. Note absence of banding although some aggregation (shading).

Lane B = ADT treated SRM. Note lack of banding and aggregation.

> 6 logs reduction in TSE infectivity

As a result of the Research conducted, ADT Rendering has the scientific data to demonstrate the levels of inactivation mentioned.